Dana T. Lounderfrom Division of Bone Marrow Transplant and Immune Deficiency, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA, and colleagues conducted a study to determine the role of circulating interleukin-22 (IL-22) levels in gastrointestinal graft-versus-host disease (GI GvHD) in pediatric patients undergoing hematopoietic stem cell transplant (HSCT). The study was publishedin Haematologica.
In order to assess whether IL-22 levels are elevated in pediatric patients with GI GvHD, the researchers analysed plasma samples obtained from 114 pediatric patients enrolled in a prospective cohort study, the Cincinnati Children’s Hospital Medical Center BMT repository. At 30-days post-transplant, IL-22, IL-22 binding protein (IL-22BP), Reg3α, and IL-17 levels were analyzed using ELISA.
- Median age: 8 years (range, 0.4–32)
- GvHD any site: 50 (44%)
- Grade 1: 9 (18%)
- Grades 2–4: 41 (82%)
Data is given as GvHD patients with IL levels above the median vs GvHD patients with IL levels below the median
- The cumulative incidence of new onset acute GI GvHD 30 days after HSCT: 15.5% vs8%, P= 0.04
- 1-year treatment-related mortality (TRM) rate: 46.9% vs20%, P= 0.6
- IL-22BP levels negatively correlated with IL-22 levels 30 days after HSCT, r = -0.2, P= 0.03
- There was no significant association between IL-22BP levels and the development of GI GvHD
- Incidence of GI GvHD 100 days after HSCT: 32% vs14%, P= 0.03
- IL-17 levels significantly correlated with IL-22 levels 30 days after HSCT: r = 0.43, P< 0.0001
- Reg3α levels negatively correlated with IL-22 levels: r = -0.22, P= 0.02
- Reg3α levels also negatively correlated with IL-17 levels: r = -0.47, P< 0.0001
Taken together, this data indicates that IL-22 levels are elevated in patients with GI graft-versus-host disease without causing higher mortality rates in this patient population. The research group stated that because of the complex role of IL-22 as well as related molecules in the setting of post-transplant patients, further studies are required to completely clarify the method of these mechanisms.